Flow cytometry: 1/100, use 10 μL of the suggested working dilution to label 10^6 cells. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 1/10 - 1/100, This product does not require protein digestion pre-treatment of paraffin sections however, staining of paraffin embedded formalin fixed tissues may be improved in some cases by enzyme pretreatment using Trypsin. Recommended positive control tissue: Tonsil. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
缓冲液
PBS, pH 7.4, 0.09 % Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
抗原
CD43 (SPN)
(Sialophorin (SPN))
别名
CD43 / Leukosialin
背景
CD43 is a heavily glycosylated and sialylated single chain glycoprotein, expressed by all leucocytes with the exception of most B lymphocytes.Synonyms: Galactoglycoprotein, Leukocyte sialoglycoprotein, SPN, Sialophorin